Fig 1: Filamin A (FLNA) associates with synaptosomal-associated protein 23 (SNAP23) and syntaxin-11 (STX11) in human platelets upon ligation of the thrombin receptor. (A) Human platelets were activated with thrombin receptor (aka F2R)-activating peptide (10 µM) prior to solubilization with NP-40 lysis buffer. Co-immunoprecipitation (co-IP) was performed in resting and activated platelets by incubating lysates with an anti-FLNA antibody. Co-immunoprecipitates were resolved using SDS-PAGE and probed with antibodies against FLNA, STX11, and SNAP23. Co-IP data indicate an association between FLNA and SNAP23 upon thrombin receptor ligation. The negative control co-IP (IgG) is also shown. Blots are representative of 3 individual experiments, using blood from different donors. (B) Human platelets were activated with thrombin receptor-activating peptide (10 µM) prior to solubilization with NP-40 lysis buffer. Co-IP was performed in resting and activated platelets by incubating the lysates with an anti-SNAP23 antibody. Co-immunoprecipitates were resolved using SDS-PAGE and probed with antibodies against FLNA and SNAP23. The negative control co-IP (IgG) is also shown. Blots are representative of 3 individual experiments, using blood from different donors.